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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
28/03/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DE ARMAS, S.; GALVÁN, G. A.; LAPAZ, M. I.; GONZÁLEZ-BARRIOS, P.; VICENTE, E.; PIANZZOLA, M. J.; SIRI, M. I. |
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Afiliación : |
STEFANIE DE ARMAS, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY.; Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; GUILLERMO A. GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur (CRS), Facultad de Agronomía, UDELAR, Canelones, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. LAPAZ, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, uy. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; PABLO GONZÁLEZ-BARRIOS, Departamento de Biometría, Estadística y Computación. Facultad de Agronomía, UDELAR, Montevideo, UY.; CARLOS ESTEBAN VICENTE CASTRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA J. PIANZZOLA, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. SIRI, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA). |
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Título : |
Phylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Plant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE |
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DOI : |
10.1094/PDIS-06-21-1140-RE |
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Idioma : |
Inglés |
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Notas : |
Published Online:27 Mar 2022.
Corresponding author: María I. Siri; Email: msiri@fq.edu.uy. |
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Contenido : |
Abstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens. MenosAbstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves... Presentar Todo |
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Palabras claves : |
ALLIUM CEPA; CENTER ROT; PANTOEA SPP; PATHOGEN DIVERSITY. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03015naa a2200265 a 4500
001 1062926
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1094/PDIS-06-21-1140-RE$2DOI
100 1 $aDE ARMAS, S.
245 $aPhylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay.$h[electronic resource]
260 $c2022
500 $aPublished Online:27 Mar 2022. Corresponding author: María I. Siri; Email: msiri@fq.edu.uy.
520 $aAbstract Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens.
653 $aALLIUM CEPA
653 $aCENTER ROT
653 $aPANTOEA SPP
653 $aPATHOGEN DIVERSITY
700 1 $aGALVÁN, G. A.
700 1 $aLAPAZ, M. I.
700 1 $aGONZÁLEZ-BARRIOS, P.
700 1 $aVICENTE, E.
700 1 $aPIANZZOLA, M. J.
700 1 $aSIRI, M. I.
773 $tPlant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE
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| 4. |  | GONZÁLEZ, M.V.; FERREIRA, V.; SIRI, M.I.; GONZÁLEZ-ARCOS, M.; GALVÁN, G.A. Identificación de fuentes de resistencia contra Clavibacter michiganensis subsp. michiganensis en el cultivo de tomate. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2021. p. 44 Financiamiento: Programa CSIC Grupos I+D - Bacterias fitopatógenas: mecanismos de resistencia hospedera y de interacción planta-patógeno.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Treinta y Tres. |
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| 5. | | PELUFFO, S.; GONZÁLEZ IDIARTE, H.; BORGES, A.; GALVÁN, G.A.; ARBOLEYA, J. Producción de semilla de cebolla (Allium cepa L.) mediante el método semilla-bulbillo-semilla para tres cultivares de Uruguay (Abs.11, Sección: Mejoramiento genético, cultivares y Producción de semillas de hortalizas). IN: CONGRESO NACIONAL DE HORTI-FRUTICULTURA, 13. AÑO DE LA AGRICULTURA FAMILIAR DE LA FAO. "SOSTENIBILIDAD DE LA PRODUCCIÓN HORTIFRUTÍCOLA FAMILAR". 3-6 SETIEMBRE 2014, MONTEVIDEO (UY). Trabajos presentados. Montevideo (UY): INIA; SUHF, 2014. p.27| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 6. | | PORTA, B.; CONDON, F.; FRANCO, J.; IRIARTE, W.; BONNECARRERE, V.; GUIMARAENS-MOREIRA, M; VIDAL, R.; GALVAN, G.A. Genetic structure, core collection and regeneration quality in white dent corn landraces. Crop Science, v.58: 1-15, July-August 2018. Article history: Received: Dec 31, 2017 / Accepted: Mar 05, 2018 / Published: April 26, 2018.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 7. | | FORT, S.; FERREIRA, V.; MURCHIO, S.; SCHVARTZMAN, C.; GALVÁN, G.A.; VILARÓ, F.; SIRI, M.I.; DALLA RIZZA, M. Potato plants transformed with the Arabidopsis EF-Tu receptor (EFR) show restricted pathogen colonization and enhanced bacterial with resistance under conditions resembling natural field infections. [Plantas de papa transformadas con el receptor de EF-Tu (EFR) de Arabidopsis presentan una colonización restringida del patógeno y mayor resistencia a la marchitez Bacteriana bajo condiciones semejantes a la infección natural de campo]. Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. Agrociencia Uruguay 2020, v. 24, no. NE2, Article 413. DOI: 10.31285/AGRO.24.413 16 p. 2301-1548 Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020.
Comité científico editor:
Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : -- - -- |
| Biblioteca(s): INIA Treinta y Tres. |
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| 8. | | DE ARMAS, S.; GALVÁN, G. A.; LAPAZ, M. I.; GONZÁLEZ-BARRIOS, P.; VICENTE, E.; PIANZZOLA, M. J.; SIRI, M. I. Phylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay. Plant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE Published Online:27 Mar 2022.
Corresponding author: María I. Siri; Email: msiri@fq.edu.uy.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Treinta y Tres. |
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| 9. | | FERREIRA, V.; TOURNÉ, F.; EASTMAN, I.; RODRÍGUEZ-ESPERÓN, C.; RODRÍGUEZ, G.; GONZÁLEZ-ARCOS, M.; MURCHIO, S.; DALLA RIZZA, M.; VILARÓ, F.; GALVÁN, G.A.; GAIERO, P.; LARAMA, G.; GONZÁLEZ, M.; PLATERO, R.; SIRI. M.I. P22. Análisis de la comunidad bacteriana de la rizósfera de genotipos de papa con diferentes niveles de resistencia a la marchitez bacteriana. [Poster]. Posters. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 48. Financiamiento: Proyecto Fondo Vaz Ferreira (DICYT): "Microbioma y resistencia: nuevo eje de investigación en el estudio de la marchitez bacteriana de la papa causada por Ralstonia solanacearum". -- Autor correspondencia: e-mail:...| Tipo: Abstracts/Resúmenes |
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